Maslinic acid ma is a natural triterpenoid widely distributed in edible and medicinal plants and has been demonstrated to possess bioactivity. Use of maslinic acid and derivatives thereof for treatment of diseases connected with elastase inhibition, such as neuromas, erythemas, sun and heat burns, and psoriasis download pdf info publication number wo2008116959a1. Antioxidants free fulltext maslinic acid ameliorates. Maslinic acid triggers compelling antiproliferative and proapoptotic effects in different human cancer cell lines. Upadhye biodiversityandpalaeobiology,agharkarresearchinstitute,g. The role of triterpenes in the management of diabetes mellitus and. Furthermore, serum glucose, leptin, and free fatty acid concentrations significantly reduced, but the serum adiponectin concentration was higher, in the maslinic acid group than in the hfd group. Research article estimation of bioactive compound, maslinic. Fernandeznavarro and others published maslinic acid. Maslinic acid is tested initially at a dose of 200 mgkg, orally administered 60 min before and after carrageenan injection. We have previously reported that ma induces apoptotic cell death via the mitochondrial apoptotic pathway in ht29 colon cancer cells. Ma significantly suppresses the dnabinding activity of nf. Maslinic acid is a pentacyclic triterpene with antioxidant and antiinflammatory properties. Maslinic acid ma, a pentacyclic triterpene acid, is widely present in dietary plants, especially abundant in olive fruit skins and hawthorn.
Novel oleanolic and maslinic acid derivatives as a promising. B mce has not independently confirmed the accuracy of these methods. Bladder cancer is among the most aggressive human malignant carcinoma and always showed resistance to traditional chemotherapy based on dna damaging drugs. Estimation of bioactive compound, maslinic acid by hptlc. Male spraguedawley rats were orally administered either maslinic acid at 5, 10 or 25 mgkg. In vitro effects of triterpenic acids from olive leaf extracts on the mitochondrial membrane potential of promastigote stage of leishmania spp. Antioxidant effects of maslinic acid in livers, hearts and kidneys of. The concentration of maslinic acid in olive oil increases as the quality of the olive oil decreases3. Assessment of the safety of maslinic acid, a bioactive. Pdf maslinic acid is a pentacyclic triterpene found in a variety of natural sources, ranging from herbal.
Maslinic acid is gradually gaining attention as an excellent pharmacologically active product because of its premium biological properties. Maslinic acid is described to have antiproliferative and antihiv properties. Maslinic acid cas 4373415 scbt santa cruz biotechnology. Molecular mechanism of maslinic acid in targeting inflammatory pathways. Pdf maslinic acid, a natural phytoalexintype triterpene from. We subjected ethanol extract of omw to activityguided fractionation. It is a member of the group of triterpenes known as oleananes. Ischemiareperfusion irinduced inflammation is associated with enhanced leukocyte rolling, adhesion and transmigration within the. The identification and quantification of its metabolites is a requirement for a better understanding of the biological effects of this triterpene.
Maslinic acid, a natural phytoalexintype triterpene from olives a promising nutraceutical. Maslinic acid is soluble in organic solvents such as ethanol. The method was validated with respect to selectivity, linearity r 0. Maslinic acid along with its other phytoconstituents like phenolic acid, flavonoids, and ascorbic acid may act as a possible therapeutic agent for preventing hepatotoxicity caused by the oxidative stress generated due to the prooxidants like ccl 4. Maslinic acid occurs in red dates, olives, camellia, megranate and sage2. Maslinic acid is a pentacyclic triterpenoid that is olean12ene substituted by hydroxy groups at positions 2 and 3 and a carboxy group at position 28 the 2alpha,3beta stereoisomer. In vitro antidiabetic effects of maslinic acid are exhibited by increasing glycogen content and inhibiting glycogen phosphorylase activity in hepg2 cells. Hence, the chemopreventive activity was investigated on early stages of carcinogenesis induced by 1,2dimethylhydrazine dmh which is a model that mimics human sporadic colorectal cancer. However, its effect on vascular smooth muscle cells vsmc has not been explored yet. Jan 11, 2016 maslinic acid ma is a natural triterpene present in high concentrations in the waxy skin of olives.
Maslinic acid is also known as crategolic acid with the chemical name of 2. It is abundant in the cuticular lipid layer of olives. Maslinic acid in olive fruit alleviates mild knee joint pain and. The healing power of maslinic acid for your health.
Maslinic acid, a natural triterpene, induces a death receptor. M significantly suppresses the dnabinding activity of nf. B maslinic acid has also been reported to suppress nf. Apoptotic and cytostatic actions of maslinic acid in. In the present study, du145 human prostate cancer cells were cultured with 025.
As part of our ongoing investigations on the selfassembly of natural products, herein, we report the results of our detailed investigation on the selfassembly of maslinic acid in different liquids. It has been reported to show immense pharmacological and biological importance including anticancer property. Free thiol groups of proteins are mainly responsible. The solubility of maslinic acid in these solvents is approximately 0.
Maslinic acid is soluble in organic solvents such as ethanol, dmso, and dimethyl formamide. Mechanistic perspectives of maslinic acid in targeting. In this study, we found that heme oxygenase1 ho1 expression was increased in vsmcs treated with ma. Hydroxyoleanolic acid, crategolic acid cas number 437341 5. Maslinic acid induced apoptosis in bladder cancer cells. Pdf novel binding studies of human serum albumin with. Furthermore, the administration of maslinic acid significantly inhibited the serum levels of insulin and glucose compared with the hfd group. Advances in research on the preparation and biological. Variability of the method was studied by analysis of bands obtained from standard solutions of maslinic acid of di erent concentration, and ng per band on same day intraday precision and on di erent days interday precision, and rsd % was. The remaining constituents of this plantmaslinic acid, oleanolic. After a 7day acclimatization period receiving the standard diet teklad global 18% protein rodent diet, animals were randomly divided into two groups of 12 and 10 mice per group control and ma, respectively. Maslinic acid differentially exploits the mapk pathway in. Oleanolic acid content prevails with regards to erythrodiol, uvaol, ursolic and. Maslinic acid is a natural pentacyclic triterpene which can be found in various natural sources including medicinal herbs 1, 2, edible vegetables and fruits 3, 4, especially in the skin of olives.
Molecules free fulltext maslinic acid, a natural phytoalexin. Maslinic acid, a triterpene from olive, affects the. Maslinic acid as a feed additive to stimulate growth and. B activity and its downstream genes expression in pancreatic cancer cells.
Maslinic acid ma is a natural compound whose structure corresponds to a pentacyclic triterpene. Maslinic acid is a pentacyclic triterpene found in a variety of natural sources, ranging from herbal remedies used in traditional asian medicine to edible. Maslinic acid downregulated both basal and egfstimulated secretion of matrix metallopeptidase mmp9, mmp2, urokinasetype plasminogen activator, vegf, and tissue inhibitors of metalloproteinases timp1, as well as the expression of upa receptors, intercellular adhesion molecules, vascular cell adhesion molecules, and ecadherin, while increasing timp2 secretion. Pretreatment with maslinic acid significantly reduces the lpsinduced phosphorylation of i. Maslinic acid alleviates ischemiareperfusioninduced inflammation. Maslinic acid modulates secreted phospholipase a2iia spla2. B regulated osteoclastogenesis in bone marrow monocytes and inhibit tnf. Action of a pentacyclic triterpenoid, maslinic acid, against. This indicated a strong binding of fma to hsa, resulting fluorescence emission in an increase of the proteins absorbance and.
Maslinic acid which has one additional hydroxyl group compared to its parent compound oleanolic acid has lower id50 for inhibition of inflammation. However, no studies have been performed to understand the. Maslinic acid exerts a wide range of biological activities including antitumor, antidiabetic, antiobesity, antioxidant, antiinflammatory, cardioprotective, neuroprotective and antimicrobial. Maslinic acid ma, a natural compound of the triterpenoid group derived from olive, prevents the generation of proinflammatory cytokines and oxidative stress.
Maslinic acid modulates secreted phospholipase a2iia. Bioavailability and metabolism of maslinic acid, a natural. Unlike the existing drugs that damage nuclear acid molecules, maslinic acid ma displays antitumor function in various types of cancers by targeting specific intracellular signaling pathways and is regarded as a promising agent for. Maslinic acid is a pentacyclic triterpene found in plants used in traditional medicine with antidiabetic, antitumor, antioxidant and parasitostatic activities. Antiallergic triterpenes isolated from olive milled waste ncbi nih. Maslinic acid is a natural pentacyclic triterpenoid widely distributed in edible and medicinal plants with healthpromoting activities. It is isolated from olea europaea and salvia canariensis and exhibits antiinflammatory, antioxidant and antineoplastic activity. Maslinic acid is a pentacyclic triterpene found in a variety of natural sources. Maslinic acid is a compound derived from dry olivepomace oil an olive skin wax which is a byproduct of olive oil extraction. Jul 30, 2019 maslinic acid significantly decreased serum tg, tc, ldl, and free fatty acid levels and significantly increased hdl levels in hfd mice table 2. M significantly suppresses the dnabinding activity of. In all the tissues analyzed, free and esterified triterpene alcohols exhibited. Studies indicate that maslinic acid blocks the generation of nitric oxide, and inhibits the secretion of il6 and tnf. Maslinic acid induces mitochondrial apoptosis and suppresses hif1.
Oleanolic acid oa and maslinic acid ma are pentacyclic triterpenic. Jun 01, 2020 maslinic acid is an active member of pentacyclic triterpenes predominantly found in dietary plants including hawthorn berries and olive fruit skins. Maslinic acid added to the diet at doses of 25 and 250 mg kg. A stock solution may be made by dissolving the maslinic acid in an organic solvent purged with an inert gas. Growth arrest of plasmodium falciparum intraerythrocytic stages pdf paperity toggle navigation. Maslinic acid, a triterpene from olive, affects the antioxidant and. Maslinic acid is found in various natural sources, most notably in pomace olive oil, and exerts proapoptotic activities in various cancer cells in vitro. Maslinic acid, the main pentacyclic triterpene of the cuticle of olea europaea l. This research was initiated to explore the anticancer potential of maslinic acid against human neuroblastoma. Effects of oleanolic acid and maslinic acid on glucose and lipid metabolism. Maslinic acid inhibits the metastatic capacity of du145. Antiplatelet aggregation of mixtures of betulinic oleanolic and. In the last decades, and in response to an increasing interest to identify new natural molecules with beneficial effects on health, maslinic acid has been isolated not only from various plants used in traditional herbal medicine.
Notwithstanding the biological activities, there is a lack of knowledge about its safety. To evaluate the antiplatelet aggregation and cytotoxic potential of betulinic acid ba, oleanolic acid oa, maslinic acid ma and their. First selfassembly of dihydroxy triterpenoid maslinic. Free fulltext pdf articles from hundreds of disciplines, all in one place parasitostatic effect of maslinic acid. Maslinic acid, a naturally occurring dihydroxy triterpenoid having a 66666 fused pentacyclic structure, is extractable from the fruits of olive olea europaea. This observation seems to indicate that a free carboxyl group at c28 of. Hydroxyoleanoic acid, a natural triterpene, exerts a wide range of biological activities, i. The capability of tachyzoites to infect vero cells treated with 1 was affected. Pdf novel binding studies of human serum albumin with trans. Molecules free fulltext reduction of preneoplastic. Therefore, maslinic acid was orally administered to spraguedawley rats at a dose of 50 mgkg of body weight. Bulk maslinic acid 10% powder,loquat leaf extract,eriobotrya. Maslinic acid exhibits both pro and antiinflammatory properties depending on chemical structure and dose and may be useful in modulating the immune response. Supressive effect of maslinic acid from pomace olive oil on oxidative stress and cytokine production in stimulated murine macrophages.
Estimation of bioactive compound, maslinic acid by hptlc, and. Pdf maslinic acid, a natural phytoalexintype triterpene. This study suggests that fruit pulp of jujube can play a vital role in prevention of liver damage. Wo2008116959a1 use of maslinic acid and derivatives thereof. Maslinic acid and its derivatives exhibit a broad range of biological properties, such as antiinflammatory, anticancer, antidiabetic, antimicrobial, neuroprotective and hepatoprotective activities. Wo2006003226a1 use of maslinic acid as a food additive. Maslinic acid ma is a pentacyclic triterpenoid recognized for multiple therapeutic effects including. Maslinic acid reduced liver weight and liver lipid accumulation and improved hepatocyte steatosis. Mice fiveweekold male balbc mice 1921 g are housed in a conventional condition and provided with the free access to standard rodent chow and water. Download pdf info publication number wo2008116959a1. Ma has many biological and therapeutic properties related to health, including antitumor, antiinflammatory, antimicrobial, antiparasitic, antihypertensive, and antioxidant activities. The supporting information is available free of charge on the acs.
Maslinic acid suppresses osteoclastogenesis and prevents. Maslinic acid inhibits the metastatic capacity of du145 human. Protective effects of maslinic acid against alcoholinduced acute liver injury in mice. The compound has attracted much interest owing to its proven pharmacologic safety and its many biologic activities, such as. Maslinic acid targets other cancerrelated signaling pathwaysorigin cell line ic 50 m references human colorectal adenocarcinoma ht29 101. Sep 17, 2020 also, maslinic acid treatment altered the mitochondrial membrane electrochemical potential and the reactive oxygen species ros levels to cause a caspaseindependent programmed cell death. Wo2008116959a1 use of maslinic acid and derivatives. Identification in rat plasma and urine by linear trap. Maslinic acid ma is a natural triterpene present in high concentrations in the waxy skin of olives.
The objectives of the present study were to estimate bioactive compound maslinic acid by hptlc method and to evaluate in vitro antioxidant and hepatoprotective activity of eight cultivars of indian jujube. In human umbilical vein endothelial cells huvecs treated with lipopolysaccharide lps, we characterized the effects of ma on the regulation of heme oxygenase ho1, cyclooxygenase cox2, and inducible nitric oxide synthase inos. Maslinic acid protects against obesityinduced nonalcoholic. B translocation to nucleus and phosphorylation of i. Expression in a549 lung cancer cells under normoxic and hypoxic conditions. Furthermore, ma was found to induce akt activation in a dose. In silico approaches and immunoblotting suggested the involvement of the mapk pathway explaining the variability in cell cycle progression along with the. Maslinic acid can be found in many cosmetic formulas due to its antioxidant and antiinflammatory effects. Jul 30, 2019 maslinic acid reduced liver weight and liver lipid accumulation and improved hepatocyte steatosis. More specifically, the invention relates to the use of maslinic acid 2alpha,3betadihydroxy28carboxyolean12ene, the derivatives or natural products thereof or concentrates of same which contain said acid, in the preparation, either alone or combined with other agents, of products that can be used in.
Therefore, the purpose of this study was to evaluate whether high. Maslinic acid protects vascular smooth muscle cells from. Pdf mechanistic perspectives of maslinic acid in targeting. Estimation of bioactive compound, maslinic acid by hptlc, and evaluation of hepatoprotective activity on fruit pulp of ziziphus jujuba mill. Maslinic acid, a natural triterpene, induces a death. Action of a pentacyclic triterpenoid, maslinic acid. The invention relates to the use of maslinic acid as an additive for animal production.
Maslinic acid as an effective anticancer agent lin. Maslinic acid 2alpha, 3betadihydroxiolean12en28oic acid is a triterpenoid compound present in fruit and leaves of olea europaea that can be used as an additive in the diet of trout. Maslinic acid modulates secreted phospholipase a 2iia spla 2iiamediated in. As an antioxidant, maslinic acid provides free radical fighting properties that aid in protecting and repair skin by deeply penetrating the dermal layers. In vitro study shows that maslinic acid inhibits serine proteases, key enzymes necessary for the spread of hiv within an individuals body. You will always find maslinic acid that are suitable for your necessities because they come in different sizes and packaging designs. The compound has attracted much interest owing to its proven pharmacologic safety and its many biologic activities, such as anti. The pentacyclic triterpenes ursolic acid ua, oleanolic acid oa and maslinic acid. It is a common element in the diet where olive fruit and olive oil are included ferna. The amount of maslinic acid in table olives constitutes 0. Pdf maslinic acid, a triterpene from olive, affects the. Here, we show that ma induces apoptosis in caco2 colon cancer cells via the extrinsic apoptotic pathway in a dosedependent manner.
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