Ion torrents new ion s5 and s5 xl systems integrate seamlessly into. Samsung galaxy s5 manual user guide and instructions. Agriculture resource guide enigma saintia solusindo. Modified pgm protocols for improved ngs data output ion. Comparison of illumina miseq and the ion torrent pgm and s5. Man0017528 provides reference information for using and maintaining the ion genestudio s5 cat. A27767 for use with ion s5 system new in box as pictured. Man0010811 provides reference information for using the ion torrent s5or ion torrent s5xl sequencers cat. The ion s5 and ion s5 xl systems come with loadandgo reagents and a straightforward user interface, so you can set up the sequencer with less than 15 minutes of handson time. Learn more at ion torrentnextgenerationsequencingworkflow ion t.
This equipment has been tested and found to comply with iec 60825. Targeted sequencing for all on ion genestudiotm s5. Automated ion ampliseq library preparation, template preparation, and chip loading cartridgebased reagent system sample and reagenttracking capability intuitive user interface onboard diagnostics simplify the ion torrent ngs workflow. The ion s5 system leverages the speed of semiconductor sequencing with impressive onboard computing power, to enable the production of high quality sequencing data in as little as 2. Charging 8 hours before using is usually copied from old user manual when nimh battery dominated many, many years back. User guide ion s5 and ion s5 xl instrument catalog numbers a27211, a272 publication number man0010811 revision a. The assay is compatible with ion pgm and ion genestudio s5 sequencing systems ion 318 ion 530 chips, with an option to use manual or ion chef library preparation and generate a comprehensive variant annotation report using our oncomine reporter. In this document are contains instructions and explanations on everything from setting up the device for the first time for users who still didnt understand about the basic function of the phone. In the kits tab, for template kit, select onetouch figure 3. The ion torrent s5 and ion torrent s5 xl instrument user guide pub. About thermo fisher scientific thermo fisher scientific inc. The first section of this manual provides background information on ion architecture and its modular structure. We have performed our first two real runs on our new ion torrent s5, and i am very happy with our ngs platform from an operational standpoint. Proton sequencer, ion s5 sequencer, ion s5 xl sequencer, or ion genestudio s5 series sequencer.
Ion 540kit ot2 ion onetouch2 system ion s5system ion s5xl system. View online or download thermo scientific ion torrent s5 user manual. The ion s5 nextgeneration sequencing system enables a simple targeted sequencing workflow for your lab at an. The ion s5 nextgeneration sequencing system enables a simple targeted sequencing workflow for your lab at an affordable price. Ion s5 and ion s5 xl systems thermo fisher scientific. Ion s5 and ion s5 xl system site preparation guide pub. The ion torrent team rolled out a new sequencer line this morning, the s5. Throughout the user guide, room temperature is 2224c. Using a high definition and intuitive touchscreen, the user is guided through the necessary steps to prepare the instrument for sequencing and to start a sequencing run. In this guide, ion genestudio s5 sequencer or system refers generically to the series of three ion genestudio s5 sequencers or systems, unless otherwise specified. Modified pgm protocols for improved ngs data output ion torrent.
Ion chefsystem ion s5system ion s5xl system ion genestudios5 system ion genestudios5 plus system ion genestudios5 prime system. Ion genestudiotm s5 series ion genestudiotm s5 ion genestudiotm s5 plus ion genestudiotm s5 prime ion 510 chip 23 m reads up to 400 bp ion 520 chip 36 m reads up to 600 bp up to 200 ion 530 chip 1520 m reads up to 600 bp ion 540 chip 6080 m reads up to 200 bp ion 550 chip 100 m reads bp fast. See genomic dna extraction kits on page 16 for a list of recommended genomic dna extraction kits. Thermo scientific ion torrent s5 manuals manualslib. In this user guide, ion genestudio s5 sequencer or system refers generically to. Ion torrent s5 andion torrent s5 xl instrument user guide 5 confidential for life technologies internal use only.
The information in this guide is subject to change without notice. Thermo scientific ion genestudio s5 user manual pdf download. Related manuals for thermo scientific ion torrent s5. The subsequent sections detail ion module specifications and configuration. You can also track your consumables with ease and accuracy with the automated radio frequency identification rfid tracking feature of the systems. Verify that the reagents have not been freezethawed multiple times and have not remained at room temperature for too long. With flexibility powered by the ability to choose from five ion torrent chips, these. Our dna fragment analysis by capillary electrophoresis user guide is a handbook for. Panasonic kvs5076h operating manual pdf download manualslib. Ion torrent s5andion torrent s5xl instrument user guide 5.
Using a fingerprint or a pin, windows hello is a fast, secure way to unlock your windows devices without entering a password. The ion s5 and ion s5 xl systems provide the simplest dnatodata workflow for targeted sequencing with industryleading speed and affordability. Equipped with a storagecharging stand, two different attachments and an extendable dust cup, the s5 is perfect for cleaning things like cabinets, window sills. The ion genestudio s5 series is a new line of benchtop nextgeneration sequencing ngs systems that enable you to efficiently run small and large projects across multiple research applications, with the simplest sampletodata ngs workflow and superior speed. This user guide describes how to use the ion torrent ion 540 kit chef cat.
The system includes a complete set of cartridgebased consumables and reagents that enable a user to load two chips in 18 hours, depending on the panel and sequencing chip, with less than 15 minutes of handson time. In this document are contains instructions and explanations on everything from setting up the device for the first time for users who still didnt understand. The ion s5 and ion s5 xl systems come with loadandgo reagents and a straightforward user interface, so you can set up the sequencer in less than 15 minutes of handson time. If the template is prepared by ion onetouch instead of ion chef. The thermo ion s5 sequencer comes partnered with a one touch 2 system to help automate and add enrichment to your workflow. Supports sequencing preparation for the ion genestudio s5 systems the ion chef system features. Thermo fisher scientifics line of ion torrent sequencers utilize ion semiconductor chips that contain million of microwells, each layered underneath with an ion sensor to measure changes in ph. However, 11 out of 12 cegenerated profiles showed more alleles for the codis loci. Approach s5 owners manual 1 \376\377measuring a shot, page\2403 \376\377keeping score, page\2402 \376\377page\2402 \376\377using courseview, page\2402. Download thermo scientific ion torrent s5 user manual. Utility of the ion s5 and miseq fgx sequencing platforms to. Laboratory equipment thermo scientific ion torrent genexus integrated sequencer user manual 163 pages. Ion torrent s5 laboratory equipment pdf manual download.
Ion torrent s5 andion torrent s5 xl instrument user guide fcc id. Samsung galaxy s5 manual user guide is a pdf file to discuss ways manuals for the galaxy s5. Trek bike owners manuals and bontrager product manuals trek. The ion s5 system and the ion s5 xl system are no longer available for purchase and are replaced by the ion genestudio s5 system and ion genestudio s5 prime system, respectively. Ion genestudio s5 instrument thermo fisher scientific. The ion s5 and ion s5 xl systems provide the simplest. Page 1 ion genestudio s5 food protection instrument user guide catalog numbers a395 publication number man0017965 revision a. Dec 06, 20 ion library quantitation kit user guide 5 kit contents and storage component ion library taqman qpcr mix, 2x amount 2 x 1. Nov 01, 2019 subsequently, the sequencing was performed on the ion s5 system thermo fisher scientific using the ion s5 precision id sequencing kit following the manufacturers recommendations. Other sequencing parameters set up on ion s5 should be the same as above described with the ion chef. In order to autorun on ion genestudio s5 system ion proton system reports, the plugin must include composite in its runtypes specification. Trek bike owners manuals and bontrager product manuals. The trimble s5 total station is a class 2 laser product the instrument contains visible and invisible laser sources a laser diode for the distance measuring function operating at 905 nm infrared, non. Precision id panels with ion s5system application guide 33.
The simplicity s5 cordless hand vacuum will become your new obsession. Thermo fisher scientific introduces ion genestudio s5 series. Care and maintenance cleaning the ion outlets and sensors remove dust from the ion outlets a with a cotton swab. The ion s5 system is simple to use with cartridgebased reagents and offers superior scalability and flexibility to support a broad range of. The ion s5 system is one of thermo fisher scientifics higher end models of next generation sequencers, utilizing the ion torrent superconductor technology to support a broad range of sequencing applications. The new systems require just 15 minutes of manual setup time per sequencing run and less than 45 minutes of handson work from dna to data when using the ion chef system for automated ion. The ion genestudio s5 prime system is a semiconductorbased nextgeneration sequencing ngs system that enables simple targeted sequencing workflows. Pdf 620 kb 2014 trek speed concept service manual english. You can also track your consumables with ease and accuracy with the automated radiofrequency identification rfid tracking feature of the systems. The s5, whose impending release had been tipped on the internet by the leak of a manual, arrives in two models, the standard and the xl, which differ only by onboard computing power and not sequencing metrics. Page 55 care and maintenance location of reflectors reflectors operating manual. Please read the instructions for use carefully prior to starting the assay procedure and. Instrument user guide that includes instructions for instrument operation and.
Str typing workflows ion torrent s5 xl sequencing platform. Trimble s5 total station user guide iii safety information laser safety before using the instrument, make sure that you understand this user guide, as well as all equipment and job site safety requirements. The instrument has three chip options, capable of producing up to 6080 million reads with read lengths of 200 to 400 bases and up to 15. Li ion battery does not require charging before using if the battery has more than about 1520% battery left. Introducing the ion genestudio s5 series for nextgeneration. Comparison of illumina miseq and the ion torrent pgm and. Page 1 user guide ion torrent s5 and ion torrent s5 sequencer catalog numbers a27211, a272 publication number man0010811 revision a. View and download thermo scientific ion torrent s5 user manual online. The ion s5 system is simple to use with cartridgebased reagents and offers superior scalability and. See the important safety and product information guide in the product box for product warnings and other important information.
Thermo scientific ion genestudio s5 user manual pdf. Thermo scientific ion torrent s5 user manual pdf download. Instructions for automated template preparation, chip loading, and sequencing for use with. Galaxy book ion s improved security features are backed by windows hello. For core and service labs requiring maximum throughput and rapid turnaround time from a benchtop ngs system, the ion genestudio s5 prime system enables unparalleled breadth and depth, from large panels or exomes to clinical oncology research focused on low allele frequency applications. A27211 or a272 is uncrated, installation and testing of the ion s5 sequencer or ion s5 xl sequencer and torrent server required for use with the ion s5 xl sequencer takes 8 hrs.
The ion s5 system and the ion s5 xl system are no longer available for purchase and are replaced by the ion genestudio s5 system and ion genestudio s5 prime system. Describes how to generate reports in oncomine reporter software. Flows enter the appropriate number of flows for the sequencing kit and read length. Describes the torrent suite software and provides procedures for common tasks oncomine childhood cancer research assay user guide man0017117. Getting started with plugins ion torrent sdk documentation. Instructions for automated template preparation, chip loading, and sequencing. With that, we can see how the ion s5 xl is a great choice for labs that either need results within 24 hours andor have a high weekly throughput. Man0010811 provides reference information for using the ion torrent s5 or ion torrent s5 xl sequencers cat. A27759, a30011 and the ion chefsystem to prepare enriched, templatepositive ion sphereparticles isps for up to 200 baseread sequencing of libraries on the ion s5.
With a thin cotton swab, remove dust from the ion outlets, and then wipe the center of the inner parts approximately 5 times. The ion s5 and ion s5 xl instrument user guide pub. The ion torrent s5and ion torrent s5xl instrument user guide pub. Use 1 ng of input genomic dna per target amplification reaction for all precision id panels. As has been the trend, ion s focus is entirely on focused sequencing, and the new lineup emphasizes making.
Thermo fisher scientific ion torrent ion s5 sequencing. Rapid sequencing of microorganisms with the versatile ion. Select the instrument and kit used to prepare the templated ion ion sphere particles. The raw data were extracted from the s5 torrent server v5. Download ion library quantitation kit user guide pdf. Life technologies corporation 5781 van allen way carlsbad, ca 92008 the information in this guide is subject to change without notice. Confidential for life technologies internal use only. Template preparation and sequencing using the ion chef and ion s5. Ce results demonstrated 2 out of 12 samples ion s5 and 7 out of 12 samples miseq with greater profile completeness than the mps systems.
Ion is part of an elite family of hardware and software companies known as inmusic brands. This user guide describes how to use the ion torrention 540kit chef cat. Ion s5 and ion s5 xl instrument user guide manualzz. These products may be covered by one or more limited use label licenses. Fragment libraries were sequenced on the ion 520 chip. Validation and optimization of the ion torrent s5 xl sequencer and oncomine workflow for brca1 and brca2 genetic testing saeam shin 1 2, yoonjung kim 1, seoung chul oh 1, nae yu 1, seungtae lee 1, jong rak choi 1, kyunga lee 1. Instructions for template preparation, chip loading, and sequencing. The extra computing power of the ion s5 xl is realized during the data analysis stage that can be completed in just 15 hours, which is 34x faster than the ion s5. This next generation sequencing system is simple to use with an intuitive interface, and a cartridgebased system, allowing users to scale up and remain flexible in the types of sequencing they can accomplish, from exomes and transcriptomes. Adapting sureselect enrichment protocol to the ion torrent. Summary of contents for thermo scientific ion genestudio s5 page 1 ion genestudio s5 food protection instrument user guide catalog numbers a395 publication number man0017965 revision a.
Sara alvarez medical director, nimgenetics, spain sequencing run times of 2. Included in box 4x bottles of ion s5 wash solution 31091 1x bottle of ion s5 cleaning solution. Man0010811 provides reference information for using the ion s5or ion s5xl sequencer cat. This allows you to spend less time doing repetitive lab work, and more time answering the critical questions in your research.
Unlike the ion pgm and ion proton, there is no need for a gas supply or specific water system to be in. Ion digital camera 4k quick start manual 11 pages 2. Thermo fisher scientific introduces ion genestudio s5. The ion chef instrument or ion onetouch 2 instrument plus ion onetouch es. Describes how to use ion reporter software for data analysis of the oncomine childhood cancer research assay. Validation and optimization of the ion torrent s5 xl. Ion torrents new ion s5 and s5 xl systems integrate seamlessly into your work. The ion s5 and ion s5 xl sequencers, along with the ion chef system, offer reproducible sequencing workflows, with minimal handson time. Samsung galaxy s5 android lollipop user guide pdf samsung galaxy s5 user manual kitkat howto guides. Oncomine myeloid research assay thermo fisher scientific us. Ion torrent s5 andion torrent s5 xl instrument user guide.
The ion one touch 2 consists of two modules, a one touch 2 intrument and an enrichment system. Ngs sequencing supporttroubleshooting thermo fisher. Describes how to use ion reporter software for data analysis of the ion reproseq assay. Ion s5 and ion s5 xl benchtop systems science aaas. This onetouch 2 system provides template amplification by integrating multiple manual steps into a single system.
Samsung galaxy s5 smg900f, smg900i, smg900kg900lg900s, smg900m, smg900a, smg900t, smg900w8 manual user guide is a pdf file to discuss ways manuals for the samsung galaxy s5. Pilot study for forensic evaluations of the precision id. With the ion s5 ion s5 xl instrument, ion sphere test fragments are not present in the test fragment report section of the run report, and library sequencing is poor. Mar 11, 2016 ion torrents ngs technology has been extremely useful for our genomics core facility, but i am convinced that the pgms current protocols for isp enrichment and other aspects of the sequencing. The ion s5 is suitable for sequencing whole genomes, targeted gene panels, exomes, transcriptomes, metagenomes, and more. Man0010811 provides reference information for using the ion s5 or ion s5 xl sequencer cat. If no run types are defined, the plugin will be launched for thumbnail and ion pgm system reports only. The inmusic profile is where you can register products, download software titles, and access exclusive content and offers not just for ion, but for any brands within the inmusic network. The workflow ion s5 next generation sequencing system. The thermo ion s5 sequencer provides a simple and powerful targeted sequencer with semiconductor speed and powerful computing power for dependable results. Targeted sequencing for all on ion genestudiotm s5 sequencers.
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